Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method

EGF family growth factors consists of growth factors, such as transforming growth factor (TGF)-a, heparinbinding EGF-like growth factor (HB-EGF), amphiregulin (AR) and epiregulin, autocrine growth factors in normal human keratinocytes. HB-EGF is mitogen for epithelial cells and like other members of the EGF family, HB-EGF exerts its biological effects via interaction with the EGF receptor (EGFR). HB-EGF is an autocrine growth factor for human keratinocytes, and has a possible role as a paracrine growth factor for fibroblast. Our report concerning immunohistochemical localization of HB-EGF in normal skin by using the streptavidinperoxidase (HRP) conjugate method, confirms previous data, revealing specific patterns of HB-EGF localization. Identification of HBEGF in cells of epithelial origin suggests its autocrine and/or paracrine roles in epithelial cell maintenance. Our report especially wants to give a technical contribution, easy to manage and with evident results. A simple technique that does not require use of sophisticated equipment.